雌性安徽白山羊胸腺形态学增龄性变化

doi:10.11843/j.issn.0366-6964.2018.08.020

Abstract

Abstract:

In order to study the age-related histomorphological changes in the thymus of female Anhui white goats at different stages of growth, 3 female Anhuai white goats in 4 growth periods(infancy, prepuberty, puberty, adulthood) were selected, respectively; and the thymus general indexes were recorded. Hematoxylin-eosin (H.E.) staining was used to observe the morphological structure of the thymus; immunohistochemistry was used to explore the distribution of S100 and Caspase-3 in thymus; in addition, the transcription levels of S100, CASP-3, CASP-8 and TP53 mRNA in thymus were detected using real-time fluorescent PCR (RT-PCR). The results were as follows:1) The length, weight, index and maximum width of the thymus reached their peaks in goats at prepuberty or puberty. 2) The boundaries between the cortex and medulla in the thymus were clear in female goats at prepuberty, puberty and adulthood, but not in infancy. 3) Caspase-3 positive cells were concentrated at the cortex and medulla of the thymus, but S100 was mainly distributed in the junction of the cortex and medulla. 4) No significant difference was found in S100 mRNA levels between different periods (P>0.05); CASP-3 and CASP-8 mRNA levels were highest at prepuberty, and TP53 mRNA was the highest at adulthood of female goats. The results indicated that female goats' thymuses showed age-related deterioration after puberty. CASP-3 and CASP-8 may participate in thymus development, cell renewal and involution processes in the early periods of development. Additionally, CASP-8 and TP53 play important role in thymus involution during adulthood.

CLC Number:

S827
S854.4

JIANG Shu-dong, ZHANG Xing-wang, FANG Fu-gui, ZHOU Wei-juan, WANG Deng-feng, ZHU Hui. Age-related Thymic Morphology Changes in the Female Anhui White Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1743-1751.

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Age-related Thymic Morphology Changes in the Female Anhui White Goat

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